解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The activation of adenosine-5'-monophosphate aminohydrolase from rabbit skeletal muscle by H+ has been demonstrated. Evidence is presented which indicates that the binding of H+ and K+ is linked, in that the dissociation constant (KA) for K+ activation is reduced as the pH is lowered. Concomitantly, the pK of several ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00641a013
更新日期:1977-11-01 00:00:00
abstract::We have investigated the effects of mixed Na+: Mg2+ ionic solutions on the stability of the nonspecific lac repressor-DNA complex. The effects of Mg2+ are simply interpreted in terms of its role as a competitor (with repressor) for DNA sites. From these studies, the binding constant of the Mg-DNA complex can be determ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00641a005
更新日期:1977-11-01 00:00:00
abstract::A statistical mechanical partition function for phospholipid bilayers is constructed to obtain a theoretical description of the chain melting phase transition in lipid bilayer membranes and of the phase diagrams for two-component bilayers. In addition to providing an accurate representation of the transition temperatu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00639a004
更新日期:1977-10-04 00:00:00
abstract::Treatment of human lymphoid cells with methyl methanesulfonate (MMS) results in discontinuities in cellular DNA. Discontinuities can be detected by sedimentation through neutral sucrose gradients after digestion with S1 nuclease on top of the gradient and by benzoylated naphthoylated diethylaminoethylcellulose (BND-ce...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00639a024
更新日期:1977-10-04 00:00:00
abstract::The chemical and enzymatic syntheses of bacteriophage lambda pseudo operator DNA are described. The 17 base-paired duplex contains the DNA which has been proposed as the binding site for cI repressor protein. The synthetic duplex is twofold symmetric and represents the best possible nucleotide summation of the six pro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00638a013
更新日期:1977-09-20 00:00:00
abstract::The sialic acid binding lectin, limulin, was isolated by gel filtration and ion-exchange chromatography from the hemolymph of Limulus polyphemus. When the purified protein was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of beta-mercaptoethanol, two major protein bands wer...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00638a026
更新日期:1977-09-20 00:00:00
abstract::Analysis of [gamma-32P]ATP in vitro labeled nuclear RNA shows transfer of gamma-32P from ATP to form the 5'-terminal monophosphate for large RNA molecules. This finding is an actively transcribing nuclear system capable of guanylation and methylation reactions indicates that polynucleotide kinase activity in the eukar...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00638a016
更新日期:1977-09-20 00:00:00
abstract::Two major neutral glyceroglucolipids (A and B) have been isolated from lipid extract of human gastric content by the procedure involving column fractionation on DEAE-Sephadex, silicic acid, and thin-layer chromatography. Both glycolipids contained glucose, glyceryl ethers, and fatty acids. The structures of these glyc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00637a003
更新日期:1977-09-06 00:00:00
abstract::Temperature-jump experiments were performed with di-, tetra-, and hexasaccharides derived from type III pneumococcal polysaccharide using a homogeneous corresponding antibody IgG 45-394. A decrease in stability of the oligosaccharide-antibody complexes with decreasing chain length was observed and entirely reflected i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00637a023
更新日期:1977-09-06 00:00:00
abstract::It had been reported previously that the beta2 subunit of Escherichia coli tryptophan synthetase [L-serinehydrolyase (adding indole) EC 4.2.1.20] can be cleaved by trypsin into a nearly functional dimeric protein, the monomer of which consists of two large, nonoverlapping, polypeptide fragments. In the present paper, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00637a012
更新日期:1977-09-06 00:00:00
abstract::Four murine myeloma immunoglobulins, A4, A47N, U61, and E109, have been studied for their binding affinities with inulin and a series of oligosaccharides derived from inulin. The results indicate that the combining site of these immunoglobulins shows highest complementarity for a trifructofuranosyl sequence (A4 and A4...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00636a005
更新日期:1977-08-23 00:00:00
abstract::The interaction of bleomycin A2 with DNA has been examined by fluorescence spectroscopy and proton magnetic resonance techniques. Fluorescence bands observed at 353 and 405 nm in the spectrum of bleomycin were assigned to the bithiazole and 4-aminopyrimidine rings, respectively. Quenching of bithiazole fluorescence by...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00635a021
更新日期:1977-08-09 00:00:00
abstract::Whole tRNA preparation obtained from a human cell line (HT-29) of colon carcinoma and purified specific Escherichia coli tRNA were reacted with pyridoxal 5'-phosphate, reduced by sodium borohydride and digested with RNase A and snake venom phosphodiesterase. Two-dimensional chromatography of the pyridoxal 5'-phosphate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00635a033
更新日期:1977-08-09 00:00:00
abstract::The primary structure of the J chain from a human Waldenströms IgM protein has been determined using a combination of automated and conventional Edman degradative procedures. Eighty-five percent of the sequence was established with peptides isolated from tryptic digests of carboxyamidomethylated and citraconylated J c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00635a002
更新日期:1977-08-09 00:00:00
abstract::The quaternary structures of Escherichia coli DNA-dependent RNA polymerase holenzyme (alpha 2 beta beta' sigma) and core enzyme (alpha 2 beta beta') have been investigated by chemical cross-linking with a cleavable bifunctional reagent, methyl 4-mercaptobutyrimidate, and noncleavable reagents, dimethyl suberimidate an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00634a008
更新日期:1977-07-26 00:00:00
abstract::Enterokinase has been isolated from the contents of bovine duodena and purified 1200-fold in 41% yield. The isolation procedure employed DEAE chromatography, affinity chromatography on immobilized p-aminobenzamidine, and gel filtration. The resultant pure enzyme exhibits a single band on sodium dodecyl sulfate gel ele...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00634a011
更新日期:1977-07-26 00:00:00
abstract::The 270-MHz 1H nuclear magnetic resonance spectra of the apo, copper(I)-zinc(II), and copper(II)-zinc(II) forms of bovine erythrocyte superoxide dismutase (EC 1.15.1.1) are reported, and assignments of resonances to ten amino acid residues are proposed. The data require that at least four and probably six histidine re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00633a003
更新日期:1977-07-12 00:00:00
abstract::A complex derived from chromatin containing one molecule of each of histones H2A, H2B, H3, and H4, termed core protein, was studied by 13C and 1H nuclear magnetic resonance. 13C line widths, when analyzed and compared with those of native and thermally unfolded representative globular proteins, showed that regions of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00632a008
更新日期:1977-06-28 00:00:00
abstract::In aqueous glycerol solutions, the rate constant for the formation of compound I of horseradish peroxidase from hydrogen peroxide is independent of the viscosity. However, whem m-chloroperbenzoic acid is used to form compound I, the rate is viscosity dependent, indicating that this reaction is diffusion controlled. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00632a023
更新日期:1977-06-28 00:00:00
abstract::The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc content of the enzy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00632a012
更新日期:1977-06-28 00:00:00
abstract::Intracellular poliovirus-specific RNA species can be measured directly by electrophoresis of total cytoplasmic nucleic acids through 1% agarose gels, resulting in the separation of single- and double-stranded forms of poliovirus RNA from each other and from HeLa cell 28S ribosomal RNA. Single-stranded RNA molecules di...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00631a027
更新日期:1977-06-14 00:00:00
abstract::The three subunits I, II, and III of bovine procarboxypeptidase A separated by reversible dimethylmaleylation can reassociate to form the reconstituted complexes I + II, I + III, and I + II + III. Since the association II + III is not possible, subunit I appears to play a central role in the formation of the complex. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00630a028
更新日期:1977-05-31 00:00:00
abstract::A previously unknown polypeptide myotoxin, designated myotoxin a, was isolated for the first time from prairie rattlesnake (Crotalus viridis viridis) venom. Electrophoretic homogeneity of myotoxin a was shown in beta-alanine disc gel polyacrylamide gel electrophoresis and in isoelectric focusing gel electrophoresis. M...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00630a035
更新日期:1977-05-31 00:00:00
abstract::Isotope exchange studies show that beef liver fructokinase has a random kinetic mechanism in which release of fructose from the enzyme is slower than that catalytic reaction. The stickiness of fructose in the presence of MgATP is confirmed by isotope partition studies, which show it to be released 0.53 times as fast a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00629a021
更新日期:1977-05-17 00:00:00
abstract::Structural properties of heparan sulfate preparations from hog mucosa and beef lung sources were obtained by application of Smith degradation and nitrous acid reactions. Products formed by these reactions indicated that most of the iduronic acid present in these mucopolysaccharides is ester sulfated, whereas N-sulfate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00629a014
更新日期:1977-05-17 00:00:00
abstract::The oxygen enzymatically inserted as a hydroxyl function by chick kidney homogenate into the 24 position of 25-hydroxyvitamin D3 to give 24,25-dihydroxyvitamin D3 is derived exclusively from 18O2. Therefore, like the 25-hydroxyvitamin D3-lalpha-hydroxylase system, the 25-hydroxyvitamin D3-24-hydroxylase system is also...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00629a015
更新日期:1977-05-17 00:00:00
abstract::Factor XII was purified approximately 14 000-fold from bovine plasma by ammonium sulfate fractionation followed by heparin-agarose, DEAE-Sephadex, CM-cellulose, arginine-agarose, and benzamidine-agarose column chromatography. By this method, about 15 mg of protein was purified from 15 L of plasma with an overall yield...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00629a036
更新日期:1977-05-17 00:00:00
abstract::A plasminogen activator secreted by cultured human pancreatic carcinoma (Mia PaCa-2) cells has been purified to apparent homogeneity by procedures including Sepharose-L-arginine methyl ester affinity chromatography, Sephadex G-200 gel filtration, isoelectric focusing, and sodium dodecyl sulfate gel electrophoresis. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00628a023
更新日期:1977-05-03 00:00:00
abstract::The technique of quasi-elastic light scattering was used to measure the translational diffusion coefficient, D, of purified human prothrombin in buffered aqueous solutions and to monitor for the first time the fragmentation of this protein as it is converted to thrombin. The values of D20,w, measured at two different ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00628a016
更新日期:1977-05-03 00:00:00
abstract::Pea histones H2a, H2b, H3, and H4 have been isolated and their interactions studied by fluorescence anisotropy, light scatter, and circular dichroism. Histones H3 and H4 are almost identical in plants and animals, but plant histones H2a and H2b differ markedly from their mammalian counterparts. Pea H2b has a molecular...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00628a009
更新日期:1977-05-03 00:00:00
abstract::Under suitable experimental conditions the aerobic oxidation of NADH catalyzed by horseradish peroxidase occurred in four characteristic phases: initial burst, induction phase, steady state, and termination. A trace amount of H2O2 present in the NADH solution brought about initial burst in the formation of oxyperoxida...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00628a024
更新日期:1977-05-03 00:00:00
abstract::After protection of cysteine-45 and -82 with iodoacetamide or N-ethylmaleimide, and in the presence of saturating concentrations of substrates, the supernatant isozyme of pig heart aspartate transaminase has been covalently modified at cysteine-390 with 3-bromo-1,1,1-trifluoropropanone. The modified enzyme retains 60-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00627a004
更新日期:1977-04-19 00:00:00
abstract::The effects of solution variables on the in vitro reconstitution of calf brain tubulin, purified by the method of Weisenberg et al. (Weisenberg, R. C., Borisy, G. G., and Taylor, E. W. (1968), Biochemistry 7, 4466-4479; Weisenberg, R. C., and Timasheff, S. N. (1970), Biochemistry 9, 4110-4116), as modified by Lee et a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00627a037
更新日期:1977-04-19 00:00:00
abstract::The lipid fatty acid composition of the cytoplasmic membranes of Escherichia coli can be varied by growing an unsaturated fatty acid auxotroph in the presence of different fatty acid supplements. Electron spin resonance (ESR) studies of spin-label partitioning into the cytoplasmic membranes of different lipid fatty ac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00627a015
更新日期:1977-04-19 00:00:00
abstract::Direct chemical titrations of reduced, purified cytochrome aa3 were monitored at 604 nm. Anaerobic oxidation by potassium ferricyanide or 1,1'-bis(hydroxymethyl)-ferricinium ion was compared with the natural substrate, molecular oxygen. The four electrons from reduced cytochrome aa3 were donated to one oxygen molecule...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00626a014
更新日期:1977-04-05 00:00:00
abstract::The ability of the isomeric quinone metabolites of benzo[a]pyrene, benzo[a]pyrene-6,12-dione, benzo[a]pyrene-1,6-dione, and benzo[a]pyrene-3,6-dione to undergo reversible, univalent oxidation-reduction cycles involving the corresponding benzo[a]pyrenediols and intermediate semiquinone radicals has been characterized. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00626a035
更新日期:1977-04-05 00:00:00
abstract::A high-molecular-weight glycoprotein has been purified from the cervical mucus of the bonnet monkey (Macaca radiata). The glycoprotein was shown to be homogeneous by electrophoresis, sedimentation equilibrium, and N-terminal group determination, and to contain 19% protein, 19% D-galactose, 18% N-acetyl-D-galactosamine...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00626a042
更新日期:1977-04-05 00:00:00
abstract::The purified ATPase of sarcoplasmic reticulum was covalently labeled with N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine (1,5-IAEDANS) or with iodoacetamidofluorescein (IAF). In reconstituted vesicles containing both types of ATPase molecules fluorescence energy transfer was observed from the IAEDANS (donor) to t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00626a003
更新日期:1977-04-05 00:00:00
abstract::Triclinic crystals of hen egg-white lysozyme cross-linked with glutaraldehyde have been treated with various denaturants and found to be susceptible to x-ray structure analysis even after major conformational changes in the protein. Cross-linked crystals were isomorphous with the native form, and electron density diff...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00626a027
更新日期:1977-04-05 00:00:00
abstract::Calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. The amount of calcium liberated is proportional to the amount of bleaching in the range of 20 to 100% bleaching and is relatively insen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00626a025
更新日期:1977-04-05 00:00:00